Blue pippin
First, a blue Pippin (SAGE sciences) was used to size libraries before PCR amplification (elution set between 50 and 65 min, on a 2% agarose gel); second, plate barcoding was used to enable sequencing on a shared Illumina NovaSeq 6,000 S4 PE150 lane, as described in Colston-Nepali et al. (2019). The 54 DNA samples were added to a larger pool of ...A PacBio Sequencing library was constructed using the SMRTbell TM Template Prep Kit 1.0 and by following the PacBio 20 kb library protocol; the library molecules, after adapter ligation and damage repair, were size selected for 15 kb and larger using Blue Pippin instrument (Sage Sciences) by following the manufacturer's instructions. The ...
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The published protocol 19 was used with minor modifications: size selection was performed using a Blue Pippin (Sage Science). In some instances we used rnh201-RED instead of rnh201::kan (see results).Pippin. Sage Science 為專業的片段分離系統廠商，一直以來 Pippin Prep (100bp - 1.5kb)表現出卓越的分離結果，之後的Blue Pippin (100bp - 50kb) 更夾帶著Pulsed-field技術的高分辨率及回收率，令Sage Science 至今仍站穩全世界市占率最高的片段分離系統供應商，以及Blue Pippin ...The genomic DNAs were broken up by 26G Needle, and subsequently, the fragments above 10 kb were selected by Blue Pippin. After terminal repair and A-tailing, the ends of the fragments larger than 10 kb were ligated to generate the DNA library. The library sequencing was performed on a Pacbio sequel platform (Pacific Bioscience, California, USA ...Sage-Scientific Blue Pippin; Life Technologies E-Gel System; Diagenode Bioruptor Standard Sonicator; Gene Expression. Quantitative PCR (qPCR) Affymetrix GeneChip Workstation; Imaging. Typhoon 9410 GE Healthcare; Biorad Gel Doc EZ; Incubators/Shakers. Labnet Vortemp 56 incubator; Mcconnell Research Mini-grow 384 Incubator; Larger IncubatorsBluePippin Operations Manual 460013 Rev K 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anLibraries were size-selected on Blue Pippin (Sage Science, Beverly, MA, USA) instrument using marker S1, with 7 and 10 kb lower cutoff. Size selected DNA was collected and purified using AMPure PB beads (Beckman Coulter, Indianapolis, IN, USA). Sequencing primer was annealed and DNA polymerase bound to the SMRT bell templates. The libraries ...The Pippin facilitates library construction for the most popular NGS platforms. The platform features the ability to collect narrow and even fragment ...selected using the Blue Pippin (Sage Science, United Kingdom). Illumina libraries were constructed with a PCR-free method. using NEBNext End Repair, NEBNext dA-t ailing and Blunt T/A.Apple Pippin isn't a hardware profile SheepShaver emulates: SheepShaver emulates a generalized PPC Mac based on a beige PPC604 hardware profile, IIRC. It doesn't know about Pippin hardware. To emulate the Pippin, you need to use MAME, which I think has the only Pippin emulator available. Setting it up is rather complex, and you'll need the ...A chromosome-scale genome assembly of rye inbred line ‘Lo7’ provides insights into its incomplete genetic isolation from wild relatives, mechanisms of genome structural evolution and the yield ...For the MinIon and PromethIon sequencers from Oxford Nanopore, the company recommends BluePippin sizing for various protocols. This library prep workflow for both sequencing systems uses BluePippin to eliminate shorter fragments; one example of outcomes shows a whopping 255 Kb read from an E. coli experiment. There's a similar rationale for ...Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...The BluePippin is a preparative electrophoresis platform that separates and extracts DNA or protein from agarose. Using pre-cast gel cassettes, biomolecules are automatically collected in buffer according to software-input target ranges. The BluePippin also features pulsed-field electrophoresis power which enable accurate collection of kb-sized DNA. Cassettes are available for DNA size ...Due to the limited input DNA amount of 1 μg, the library was not additionally size-selected by, for example, Blue Pippin or SAGE Science. Sequencing-by-synthesis was performed on a HiSeq 3000 with 2 × 150 bp paired-end reads at the Max Planck Genome Centre Cologne, Germany.The BluePippin produces higher quality DNA, improving downstream analysis. Pre-cast agarose gel cassettes are constructed in a way that eliminates cross contamination. WHY use the BluePippin? (Advantages) The CGRB houses multiple Illumina and Pacific Biosciences next generation sequencing platforms that all have applications that benefit from ...BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.He added a second Pippin Prep as well as the longer-fragment Blue Pippin to his arsenal earlier this year. In Sharpe's lab, which also serves as a core facility for NRC and other Canadian government agencies, assembly projects tend to focus on large plant and fungal genomes. His team relies on Illumina and 454 sequencing, often adopting a ...When it comes to choosing a web host, there are many options available. One of the most popular choices is Blue Hosting. But how does it compare to other web hosts? In this article, we will explore the features and benefits of Blue Hosting ...Check out our blue pippin fabric selection for the very best in unique or custom, handmade pieces from our fabric shops.最主要的是普通的切胶回收只能回收中小片段，不能回收三代平台所需的大片段。. 因此手工切胶回收正在被其他更安全简便的方法替代。. 其次是磁珠分选，磁珠分选在二代测序中应用比较广泛。. 原理是磁珠会优先抓取较大的核酸片段，在分选过程中，通过 ...Sequencing of samples was performed using MiSeq Reagent Kit v3 (600 cycle; 2–300 bp) (Illumina, San Diego, CA, USA). For Long-read WGS, the library preparation and size selection were performed using PacBio Template Preparation 1.0 kit (Pacific Biosciences, Menlo Park, CA, USA) and Blue Pippin (Sage Sciences, Beverly, …今、PacBioを使って20kbライブラリをSequenceするときは、Blue Pippinによるサイズセレクションがほぼ必須になりつつあります。 彼らは今、NA12878のサンプルを、Blue Pippinサイズセレクションした後のライブラリを使って、読み続けています。
Sage Science Blue Pippin - Gel-based size-selection of fragments from 100bp to >30kb (depending on cassette and assay). Life Technologies Qubit 3.0 Fluorometer - Fluorescence-based quantitation using intercalating dye specific for DNA, RNA, or protein (so no contamination is included). Only 1μl of sample is required. MBCL:Applications. This popular instrument is a fluorescence reader for individual tubes that is used in conjunction with reagents kits to quantify dsDNA, ssDNA, RNA, and protein. This reader is easy to use and much more sensitive that the Nanodrop and is the method of choice for quantifying Illumina libraries or low concentration samples.Air More Uptempo 'Peace, Love, Basket …. $150+. Back. 1 of 10. Nike /. Nike Basketball /. Nike Scottie Pippen Basketball Shoes. Shop the latest Nike Scottie Pippen Basketball Shoes Sneakers and more at Flight Club, the most trusted name in authentic sneakers since 2005. International shipping available.The BluePippin is a powerful automated DNA size selection system to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular NGS platforms and is a fast and highly accurate and reproducible option over traditional gel extractions. The BluePippin works by pulsed-field electrophoresis to ...The pooled libraries were subject to size selection (425-525 bp) to remove host DNA amplification products using a Blue Pippin Prep device (Sage Science, Beverly, MA) and employing a 2% agarose gel cassette. The pooled libraries were loaded onto an Illumina MiniSeq mid-output flow cell (2x150 paired-end reads) with a 20% phiX spike in. Based ...
1. Blue Pippin Start up 1.1. Powering up the Blue Pippin. The Blue Pippin instrument has to be rebooted each time it is used in order to connect to the server. Once it is turned off it loses the connection. Either reboot or if the device is turned off prior to use, locate the black power button at the rear of Blue Pippin. Push the button and ...Pippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Blue Pippin. BluePippin. DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field(100bp - 50kb) BluePippin系統擁有如同Pippin Prep系列的DNA片段分離能力，搭配脈衝電場電泳(pulsed-field electrophoresis)技術，能在大片段DNA分離上，提供更佳的分離解析度及效率。高度通透過濾(High-pass ...…
Reader Q&A - also see RECOMMENDED ARTICLES & FAQs. User Guide BUF-7510 Sage Science Part Nos: Sage Science,. Possible cause: Bob Pippin is an Allstate Personal Financial Representative from Blue Springs, MO off.
Pippin is a 1972 musical with music and lyrics by Stephen Schwartz and book by Roger O. Hirson. Bob Fosse, who directed the original Broadway production, also contributed to the libretto.The musical uses the premise of a mysterious performance troupe, led by the Leading Player, to tell the story of Pippin, a young prince on his search for meaning and significance.“Blue balls” is slang for that uncomfortable and often painful feeling in a guy’s downstairs region, and it happens to some, not all, guys. But did you know that women can get it too? Blue balls and blue vulvas are very real, and we could a...Pippin Prep DNA Size Selection System Selects 100 bp – 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse ...
User Guide BUF-7510 Sage Science Part Nos: Sage Science, Inc. Suite 2400 500 Cummings Center Beverly, MA 01915 TM High-PassTM DNA Size Selection BLF-7510Sage Science size selecting with bluepippin Size Selecting With Bluepippin, supplied by Sage Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.
Sage Science and Blue are trademarks Pippin of Abstract Root-knot nematodes from the genus Meloidogyne are polyphagous plant endoparasites and agricultural pests of global importance. Here, we report the high-quality genome sequence of Meloidogyne luci population SI-Smartno V13. The resulting genome assembly of M. luci SI-Smartno V13 consists of 327 contigs, with an N50 contig … Pippin Hills is a boutique venue and winery located just oThe entire elution was size selected on a 3% agarose c Built to make bath-time fun and enjoyable, this fold-away design makes storage easy without compromising a full-size tub for your little one.Run times are as little as 30 minutes. To resolve very large DNA fragments, pulsed-field runs may last several hours. View the Blue Pippin cassette reference chart to explore the DNA size selection options and run times. Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase. Both approaches produced similar total yields of high-quality reads (r Blue Pippin Instrument Pulsed-field automated DNA size selection system The Sage Science product portfolio combines pre-cast agarose cassettes with a high-precision, automated solution for the accurate and targeted size-selection of DNA fragments for library construction in NGS workflows FlexibilityCassettes are disposable and pre-cast with agarose and running buffer. Samples are not optically detected.Blotting kits include all the components required to transfer protein, DNA, or RNA samples onto membranes for staining. The materials are grouped specifically for the method and the tissue specimen being implemented, allowing the entire procedure to be completed more rapidly. Using the ... This question is about the Blue Cash Everyday® Card from American EBLU0001 The BluePippin System includes insPippin Hill Farm & Vineyards Pollak Vineyards The Pippin platform has since established itself as a key platform for the construction of high-quality libraries for next-gen sequencing. In 2014 Sage Science released a new electrophoresis platform, the SageELF for whole sample fractionation of DNA or proteins, and the PippinHT , a high-throughput version of the BluePippin. The ColourTex® stainless steel range is unique w Are you looking to get the most out of your 2007 Honda Civic? The Blue Book is a great resource to help you do just that. The Blue Book is an online resource that provides information on the value of used cars, including the 2007 Honda Civi... The ColourTex® stainless steel range is unique whereby the colou[It was then centrifuged at 5000 × g for 10 min at 4°For the Blue Pippin protocol, the SMRTbell templates The DNA sample was fragmented with Megaruptor (Diagenode) to 15 kbp and size-selected (>10 kbp) using the Blue Pippin (Sage Science), and prepared for sequencing using the SMRTbell Express ...The published protocol 19 was used with minor modifications: size selection was performed using a Blue Pippin (Sage Science). In some instances we used rnh201-RED instead of rnh201::kan (see results).